When audio playback is stopped while BIAS Repli-Q is running as a plug-in, the host program may still be sending digital silence (a zero-amplitude signal) to it. If the Learn Spectrum button has been clicked, Repli-Q is learning the spectrum of the silence, which is a flat spectrum of very low amplitude (about -96dB). If playback then starts, Repli-Q continues averaging the actual signal spectrum with the -96dB spectrum. Naturally, the final spectral average will be false. The correct manner of operation is to start playback first, and then click the Learn Spectrum button in that order. Then click the Learn Spectrum button a second time to stop learning.
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