Latency refers to the delay between the time you play something and the time you hear it. For example, I play a guitar chord. Do I hear it instantly, or a few milliseconds later? All currently shipping MOTU audio interfaces have zero latency monitoring, which is accessed thru the CueMix software that ships with these interfaces. If you’re using hardware supporting direct hardware playthru (such as Digital Performer or Audiodesk) it can be accessed directly in your sequencer as well; in DP or AudioDesk, this is found under the Setup menu > Configure Audio System> Input Monitoring Mode.