Yes. Any PreSonus audio interface can be used instead of your computer’s built-in soundcard.
On a Mac:
Go to Preferences and choose Sound. In the Output device pull-down menu, select the your PreSonus interface from the list and click OK
On a PC
Go to Control Panel and choose Sounds and Audio Devices Properties. Select the Audio tab and choose your PreSonus interface from the Sound Playback Default Device pull-down menu. Click Apply and then OK.